Combined anaerobic digestion of chicken manure and corn straw: study on methanogenic potential and microbial diversity

Abstract Purpose To explore the methane production potential and microbial community changes of combined anaerobic digestion chicken manure corn straw. Increase production, reduce environmental pollution caused by burning livestock straw, provide some theoretical references for construction operation actual biogas projects. Methods Different proportions (3%, 5%, 10%) straw were added to systems in order improve C/N ratio evaluate feasibility synergistic effect on co-digestion. The key point was use 16S rDNA sequencing analyze relationship between diversity hydrolase activity during digestion. Result results showed that volumetric gas 3% addition group 227.66 ml/gVS, which 18% higher than cumulative pure experimental group. However, with increase concentration, utilization rate raw materials continued decrease. change each agreement hydrolytic acidifying bacteria, main also increased straw; correlation coefficient 0.9943. Sequencing dominant strains methanogenic archaea Methanosarcina , Methanosaeta Methanobacterium Methanospirillum . Mainly hydrogen-eating, acetic acid-eating methanogens, its role is H 2 methanol acid, other substances metabolize methane, convert it into CH 4 CO Conclusion a small amount enhanced capacity hydrogen-nutritive extent, species richness evenness improved, reducing environment while controlling burning. Graphical